application of semi-nested pcr rflp for detection and genotyping gbv-c among hbv-infected patients

objective: gbv-c is a virus transmissible via blood transfusion and sexual routes. because of the shared transmission routes in gbv-c and hepatitis b virus (hbv) we have attempted to detect gbv-c in hbsag-positive patients using the more sensitive semi-nested pcr. we used restriction fragment length polymorphism (rflp) to determine genotype. methods: a total of 100 serum samples were collected from hbsag-positive patients from 1389-1390. after designing specific primers and optimizing the semi-nested pcr, sequences of pcr products were analyzed by neb cutter software. restriction enzyme sites were determined and suitable enzymes selected for rflp. results: semi-nested pcr shows acceptable sensitivity for the detection of gbv-c and its genotype determination. this technique is more affordable than other techniques. conclusion: there appears to be a high rate of gbv-c infection among iranian hbv patients. in this study, the majority of patients co-infected with gbv-c were of hbv genotype 2, which is similar to the pattern seen in the us and europe.